What are effective methods for removal of iron from organic acids?

I am seeking methods to remove trace iron from organic acids, including acetic acid, propionic acid, and formic acid. Distillation works, but is expensive. I'm looking for alternatives to...

31 January 2021 984 3 View

How can I simulate VANET using OSM, SUMO and NS2, specifying WiMAX as the protocol/technology for the V2R communication and IEEE 802.11p for the V2V?

I am new to these network simulators (OSM, SUMO and NS2), but need to use the simulators for a project i am currently working on. i am total a beginner at these simulators, therefore, i will...

28 August 2020 2,734 5 View

When using explanatory sequential mixed methods, why do you need to use this method when the population groups are uneven?

I am currently working on my dissertation. I wanted to do a quantitative study using 3 groups. Two of the groups are approximately the same size, but the third group is significantly smaller....

01 May 2020 6,561 3 View

Anyone know if T cell depleting antibodies (Th1.1, Cd8) affect tumors or just periphery?

I'm want to deplete cells only in the periphery but do not know if they will deplete T cells infiltrating tumors

07 April 2020 1,962 3 View

What analytical tools can be used in retrospective study on impact of resistant Acinetobacter baumannii ?

21 February 2020 3,702 1 View

How do I convert a file in MATRIX format to FASTQ format?

I am analyzing this Hi-C data and juicer software would not accept it in its Matrix format. How do I convert it to a recognizable format like Fastq or .hic formats?

05 March 2019 6,475 2 View

Qualitative data set from interviews or conversation?

I'm looking for a qualitative data set (preferably one that has already been published) that has transcribed and coded from interviews and/or small group setting. The topic area is not important....

27 December 2018 6,840 6 View

Can I generate species rarefaction curves based on two different trapping techniques?

I am using two types of traps to collect insects and would like to predict the species richness of different localities. Is it possible to generate rarefaction curves for both methods combined or...

14 March 2018 553 3 View

How do I get a free copy of Primer?

Is it possible to get a free copy of Primer. If not is there another statistical software which is good for analyzing diversity indices. Thank you.

10 December 2017 6,066 4 View

Adding jpg files of paper?

I want to add information for my Fluorine Electron Microprobe Analysis talk at an 1982 conference. There are 3 jpg images of the paper. [ I'm trying to get a pdf file ] Is that possible?

25 February 2016 3,551 6 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View