I would like to perform tyrosine hydroxlase ELISA. Looking for protocols on how to isolate protein and successful kits. Do I isolate the SN/VTA or homogenize the whole brain, etc? I would ideally like to use half the brain for confocal imaging and the other half for ELISA. Not sure if that is possible considering I usually perfuse rats for imaging but don't think that would work for protein isolation. Thanks!
Hello
For protocol see these link , also there are Elise Guide
add , i suggest many ELISA kites which you can use in your experiment
Hope help you
Good Luck
http://www.jove.com/visualize/abstract/23537934/development-an-enzyme-linked-immunosorbent-assay-elisa-to-measure
http://www.biocompare.com/pfu/110627/soids/328494/ELISA_Kit/Tyrosine_Hydroxylase
http://onlinelibrary.wiley.com/doi/10.1002/jnr.490230311/abstract
https://resources.rndsystems.com/images/site/erg20106836.pdf
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