The ThermoBrite Elite system automates the pre- and post-hybridization steps in FISH testing.
How efficient is it? Is it a closed system? Is it FDA approved?
We use the smaller version ThermoBrite since many years. Only for hybridization and that works fine.
If you want pretreat tissue slides for FISH the system could be helpful. Just for regular FISH (cultured cells, smears, etc.) we have found it not efficient. It doesn't add the probe automatically and you will have to remove the coverslip by hand.
Thanks Thomas and Michael. For pre and post treatment, does it require special reagents from vendor? Or can we use our own e.g. regular xylene?
special reagents are not necessary. We used it with our own pretreatment Kit (note: I am working for a commercial probes company!). For Xylene please verify to have the right tubes. In a first trial the tubes provided where not stable and started to leak. However, after replacing it worked (for how long I dont know as we gave up).
VP2000 is also useful, just to automate the pretreatment. We dont use it because it's a little expensive for what it can do.
We have tried TB Elite for our slides. Overall, i do not recommend it for small lab use. It does not take much of hand work. For standardization purposes, it could be useful for big clinical labs which run big number of slides simultaneously. Expensive consumables as expected but you can work around this somehow. As Micheal stated before, Xylene is harmful for regular tubes.
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