using the table form the paper reproduced in wikipedia and at Sigma my pH 7.2 and 7.4 come out at 7.4 and 7.6 for the 7.2 and 7.4 target. Is it just me? checked with 2 probes
Hi everyone, we are trying to establish BN-gels in our lab and we are currently facing some problems. We would like to investigate protein-protein interaction of membrane bound proteins from cell...
18 April 2024 3,219 0 View
1.) We are conducting adsorption experiments with methylen blue onto powdered acitvated carbon (liquid batch). 2.) To ensure constant pH conditions we have worked with potassium phosphate buffer...
14 November 2023 1,232 3 View
Looking for a straightforward approach to isolate nuclei that does not require the use of an ultracentrifuge... we generally work with mitochondria, however, we are interested in examining some...
16 October 2023 9,925 1 View
I am currently working in the monitoring of pests of a group of crops. The main issue we're facing at this time is how to properly calculate the levels of each pest for which the application of a...
01 August 2023 4,689 1 View
The Pierce Borate buffer sold by Thermo Fisher: 20X Borate Buffer is ideal for preparing sodium borate buffer solutions for use in protein modification procedures requiring amine-free buffer at an...
01 August 2023 7,541 4 View
Hey there, I‘m using SPSS for the statistical synthesis of my Meta-Analysis and I can only choose log(OR), log(RR) etc as an Effect sizes. Therefore my forest plot is also on a logarithmic scale…...
19 June 2023 8,478 2 View
Hey there, I‘m currently working on a surivival meta-analysis and I use SPSS for my statistics. The problem is that I have a lot of data regarding mean OS / PFS, but no belonging standard...
01 June 2023 6,470 4 View
My email is [email protected]
21 February 2023 7,227 3 View
I am running a PCA in JASP and SPSS with the same settings, however, the PCA in SPSS shows some factors with negative value, while in JASP all of them are positive. In addition, when running a...
15 December 2022 1,540 4 View
Hi all, we would like to perform metagenomics on plant tissue as well as modern soil samples. We are not experienced with these analyses neither on plant material nor on modern soil. Has anyone...
07 November 2022 3,837 1 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View
Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...
13 July 2024 1,675 3 View