I am going to check if siRNA co-formulated with protamine and PEGylated anionic liposomes is efficiently protected from serum nucleases.

At different incubation times of liposomes with serum, aliquots will be withdrawn and the RNAse activity will be stopped by fast heating at 80°C.

The residual intact siRNA will be visualized on agarose gel by a safe view nucleic acid stain. However, to be visible in the gel, the siRNA has to be free from protamine and liposomes, which require a disassembly step prior to run the gel.

Do you think SDS would be suitable? At which concentration?

Heparin is used to disassembly siRNA-cationic molecules complexes but in my case it would probably not be able to disrupt liposomes. Is it correct?

Thanks for your time and help.