We have the old Light Cycler but need to format the PC and have lost the software CD... obviously is an obsolete machine but is the only we have :-(
Any Help????
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Because I have realized that the world tends more and more to do open and free science and there is a trend more and more to choose free databases, free tools and open access platforms.
01 August 2024 10,046 1 View
Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...
27 July 2024 5,748 0 View
Cuáles fueron las tendencias en investigaciones en arquitectura, urbanismo y patrimonio edificado en decadas del 2000 al 2020? Porque requiero conocer tesis de posgrado nivel maestría...
24 July 2024 5,494 1 View
Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you
24 July 2024 3,937 0 View
Hi, I have performed an Annexin V apoptosis study using flow cytometry and got quite good results. I doubt how to represent the quadrant percentages in the final bar graphs. Some papers only show...
13 July 2024 356 3 View
Hello dear researchers, I would like to know about available in silico tests that could predict the activity of ligands (small molecules) against the target protein. I have performed docking...
08 July 2024 4,724 4 View
seti or search of intelgenece articial nee to be fixed due to masss solar systems outhere thta expelled laser beam light ollimated wel beign so is neede to modificated the seti in a new device of...
21 June 2024 5,059 1 View
I synthesized pure ZnO with a hydrothermal microwave method and decorated them with CuO with the incipient wetness impregnation method. The Zn2p edges of pure ZnO are at 1044.7 and 1021.6 eV...
18 June 2024 9,405 2 View
One of the most intriguing frontiers in contemporary research is the application of Generative Artificial Intelligence (GenAI) in qualitative research. This technological advance offers tools that...
13 June 2024 781 2 View
Hello, I'm trying to introduce mutations into a gene via CRISPR/Cas, and I have to check the presence of mutations in a short DNA segment (about 20 bp) within a 512 bp long sequence....
06 February 2024 3,600 1 View
We are working on the expression of resistance genes in potatoes using the LightCycler ® 480. There are two ways to evaluation of the results: basic (that use ΔΔCT method) and advanced (that use...
26 November 2023 8,283 8 View
How can I download lightcycler 96 software to analyze Rosh system.
11 September 2023 1,289 0 View
Hi, in my lab we have an old lightcycler 2.0 qc system that we want to use, unfortunately we do not have the CD, does any one know were can I download the software for this machine?. thanks to...
04 June 2023 8,087 3 View
I am using Roche Lightcycler 96 machine to perform protein thermal shift assays. I added 15 uL of 50 uM protein and 5 uL of SYPRO ORANGE fluorescent dye in DMSO. The temperature ranged from 30 °C...
12 March 2023 1,251 1 View
I get an error during relative quantification analysis in Lightcycler 480 software: Error calculating subordinate analysis, index exceeds matrix dimensions. Has anyone encountered and solved this...
10 December 2022 5,983 0 View
The first few qPCR runs the primers worked and I was getting signals in my samples/positive controls. However, now when I run another qPCR with the same primers I get no signal. I've tried using a...
17 October 2022 746 5 View
I've got a Roche LightCycler that I use to run my qPCRs. It used to take 1h 37 min to run a qPCR but now it take 1h 45 min using the same exact settings. Could the problem be that the machine is...
17 October 2022 9,072 3 View
I performed a qPCR experiment using QIAGEN's miRCURY LNA RT kit and miRCURY LNA SYBR Green Kits to investigate the miRNA expression levels in some genes, but I could not obtain the desired result...
07 June 2022 1,682 0 View
Hi, Does anyone know if it is possible to transfer the results obtained from the analysis with CT value and fluorescence level of a PCR to an excel file? Thank you in advance
14 January 2022 5,286 8 View