We have the old Light Cycler but need to format the PC and have lost the software CD... obviously is an obsolete machine but is the only we have :-(
Any Help????
I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.
02 March 2021 8,156 2 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit
20 February 2021 3,366 5 View
Good evening everyone. If a questionnaire has 4 factors measuring different constructs as for example CAMI questionnaire measuring attitudes toward the mentally ill that has four factors:...
18 February 2021 3,223 3 View
Hello everyone I would like to improve the buffer capacity of seawater (pH ~8) in order to dissolve as much CO2 as possible without dropping the pH below 7.5 (a range between 7 - 7.5 is...
17 February 2021 2,797 2 View
Hi, everyone Could someone recommend papers about Particle swarm optimization (PSO technique), using MATLAB, please? Thanks in advance
03 February 2021 3,811 6 View
I am conducting a state-of-the-art analysis of these concepts. If you have contributions, please, send me.
17 January 2021 1,123 3 View
Do they accept everything or they still pass them on a sieve?
12 January 2021 3,515 15 View
In recent years our group has highlighted the importance of trees and plants in public and recreation parks in large mega cities. However, we are interested in developing "green shields" to be...
05 January 2021 6,500 3 View
Hello, everyone: I've been reading some old and new papers on how to compute beta diversity using incidence or abundance data from several assemblages/communities. For my data, I am interested...
03 January 2021 9,293 1 View
We own a small cPCR thermal cycler from Roche, Lightcycler Nano, which is convenient for backup quick runs. Recently, Roche has discontinued the plastics for it and they offer no alternative from...
17 February 2021 8,694 5 View
We are using Cobas z480 for multiplex RT-PCR using 3 taqman probes FAM/VIX/CY5. Once the PCR finish we want to see in the amplification curve the three results for each sample at the same time....
28 October 2020 8,372 1 View
Hi everyone, If anyone here uses Roche LightCycler 2.0 instrument for qPCR experiments, I would appreciate if they message me. I have some questions about it. Best - Navid
12 October 2020 6,234 2 View
The curve by B in Hex starts great but the plateau looks like zigzag. I tested different primer concentrations and different annealing temperatures. We use Lightcycler 480 II. Color compensation...
10 May 2020 7,879 3 View
Hello everybody. I have performed efficiency tests for primers of 12 genes with UPL probes in a Lightcycler 480 to obtain the curves with 4 dilutions of cDNA. I obtained optimal results for 10...
20 January 2020 2,431 1 View
31 December 2019 6,824 1 View
I am running qpcr using Ultra-fast III SYBR Green by Agilent in rocher light cycler 480. My sample is cDNA human cell line. I used reaction volume of 10 ul/20 ul. When I run, no cp value in my...
10 November 2019 2,470 2 View
Hi all, I am trying to detect the levels of RORC in my cells. I am using a probe from Merck and my housekeeping gene is GAPDH. I am getting GAPDH when I perform the qPCR, but no RORC. This...
03 April 2019 4,350 2 View
Hi, I am running standard dilutions (1:5) of some cDNA template to check the efficiency of primers. I found to have apparently PCR inhibition, as the efficiencies that I am getting for all the...
02 March 2019 6,023 4 View
If so, how long after it expired? It has been stored in -20 degrees and will expire in one month. I have ordered more than I could use for the time being. Thanks.
08 September 2018 8,958 5 View