How can I download lightcycler 96 software to analyze Rosh system.
Dear all, I am writing to explain my problem and would greatly appreciate any small hints that might help alleviate my desperation. I am relatively new to proteomics, and our laboratory has an old...
26 June 2024 9,948 10 View
There are two graphs [a] is a multi-wall carbon nanotube and [b] is a carboxylated multi-wall carbon nanotube (functionalized). I think there is a problem with the result, especially with a graph...
13 April 2024 2,623 5 View
I synthesized a solution containing Cu2+ (0.5 g/l), Fe3+ (0.26 g/l) and Fe2+ (0.19 g/l) with hydrated sulphate salts and I followed iodometric method containing Starch solution (1 %wt), Na2S2O3...
12 March 2024 4,335 1 View
I added 1mg of KO2 in 2 mL of Dimethyl sulfoxide and sonicate it about 10 minutes.
13 December 2023 1,603 2 View
I am encountering several issues in my real-time PCR experiments and seeking guidance on troubleshooting: 1. Inconsistent Amplification Curve Overlap: The amplification curves in my real-time...
08 October 2023 4,939 0 View
I prefer to get the answer for Italy or the average amount of the European Union with a valid reference. Thanks for your contribution in advance.
21 September 2023 1,247 4 View
Hello everyone I'm modeling a steel-reinforced coupling beam in DIANA which is embedded to an adjacent shear wall. I want to model rods in the embedment region, which only transfer axial loads...
17 September 2023 5,640 2 View
I recently conducted cDNA synthesis followed by conventional PCR for quality assessment. In the course of this experiment, I observed some unexpected results that have raised questions about the...
09 September 2023 1,020 5 View
Hi, I want to analyze a crack in 2d and 3d and find displacement of the Part Block due to that Crack and loading. My question is as I want to create periodic Sine load applying for several second...
21 July 2023 7,212 0 View
During the electrophoresis run on a 1% agarose gel at 100 volts for 25 minutes, I was unable to clearly visualize the expected 28S and 18S bands representing ribosomal RNA. Instead, a smear-like...
27 June 2023 532 5 View
Hello, I'm trying to introduce mutations into a gene via CRISPR/Cas, and I have to check the presence of mutations in a short DNA segment (about 20 bp) within a 512 bp long sequence....
06 February 2024 3,600 1 View
We are working on the expression of resistance genes in potatoes using the LightCycler ® 480. There are two ways to evaluation of the results: basic (that use ΔΔCT method) and advanced (that use...
26 November 2023 8,283 8 View
Hi, in my lab we have an old lightcycler 2.0 qc system that we want to use, unfortunately we do not have the CD, does any one know were can I download the software for this machine?. thanks to...
04 June 2023 8,087 3 View
I am using Roche Lightcycler 96 machine to perform protein thermal shift assays. I added 15 uL of 50 uM protein and 5 uL of SYPRO ORANGE fluorescent dye in DMSO. The temperature ranged from 30 °C...
12 March 2023 1,251 1 View
I get an error during relative quantification analysis in Lightcycler 480 software: Error calculating subordinate analysis, index exceeds matrix dimensions. Has anyone encountered and solved this...
10 December 2022 5,983 0 View
The first few qPCR runs the primers worked and I was getting signals in my samples/positive controls. However, now when I run another qPCR with the same primers I get no signal. I've tried using a...
17 October 2022 746 5 View
I've got a Roche LightCycler that I use to run my qPCRs. It used to take 1h 37 min to run a qPCR but now it take 1h 45 min using the same exact settings. Could the problem be that the machine is...
17 October 2022 9,072 3 View
I performed a qPCR experiment using QIAGEN's miRCURY LNA RT kit and miRCURY LNA SYBR Green Kits to investigate the miRNA expression levels in some genes, but I could not obtain the desired result...
07 June 2022 1,682 0 View
Hi, Does anyone know if it is possible to transfer the results obtained from the analysis with CT value and fluorescence level of a PCR to an excel file? Thank you in advance
14 January 2022 5,286 8 View
I am currently performing qPCR work on intestinal (duodenum and jejunum) tissue collected from the pig small intestine and stored at -20°C in RNALater Stabilization Solution until qPCR analysis....
12 December 2021 564 3 View