Does anybody have experience in using the dolomite microencapsulation system (http://www.dolomite-microfluidics.com/en/new-prods/droplet-systems)? I would like to embed single microbial cells (E. coli) into microspheres of approximately 20-30 micrometers for subsequent FACS analysis. Has anybody here such a system and used it for generating such bead-diameters? How much optimization is needed before getting such a system "up and running"? Any suggestions of other potential systems are welcome.
Hi Lars,
We have it here in the lab and I have given it a try. It is totally possible and I got small agarose beads with it. I didn't have time to keep working on the optimization but it is totally possible.
Hi Lars,
Have you had any luck with encapsulating cells yet? If you share your email, I could send you further information. You could alternatively send me an email at [email protected]
Within a day you could start producing droplets reliable and at high frequency. Encapsulating cells is sometimes tricky and mostly related to their fragility.
Thanks,
Ashok
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