I have isolated an Alternaria spp from barley leaf spot, all samples are pure culture, Do anyone isolate this fungus before and try to do the pathogenicity test?
Hello Oadi Manty, I have done the pathogenicity test in case of plant pathogens. You can keep the barley leafs in moisture chamber and easily apply the 8 mm disc of Alternaria on the leaf. After this Incubate the moisture chamber in BOD incubator according to the growth condition.