How to set up TETRA-ARMS PCR at higher temperatures?

Hello everyone .i'm trying to do TETRA ARMS PCR. My primers have TM :64 and one of the primers has TM :66(Reverse outer). How can I setup at higher temperatures to have mutant band? Because at 65...

03 August 2020 4,742 2 View

Can somebody help me and recommend publications for extracting Radiolarian microfossils from the Middle to Upper Permian strata?

Dear researchers, I'll be grateful if it is possible to recommend any method papers for extracting Radiolarian microfossils from the Middle to Upper Permian. Sincerely yours, Soheil.

22 March 2020 5,895 4 View

Can anyone suggest what is this microscopic fossil extracted from Middle to Upper Permian limestone strata?

Dear Researchers, I found it from Middle to Upper Permian limestone strata in Julfa NW Iran country. This microfossil extracted form sequences that belong to the Stratigraphy and bio-event...

16 February 2020 3,088 10 View

How can i control a DMD by FPGA?

I am trying to create some pattern with a DMD (digital micromirror device) and I want to configure a FPGA to drive the DMD but I'm running into some problems.

15 February 2020 9,508 3 View

Anybody knows what is this microscopic fossil from the Middle to Upper Permian limestone strata?

Dear Researchers, Anybody knows what is this microscopic fossil from the Middle to Upper Permian limestone strata? I found it from the Middle to Upper Permian limestone strata in Julfa NW Iran...

26 January 2020 3,108 4 View

Is the water passing through the nafion?

i want to use nafion for desalination.i have a setup that contains two continuous streams of salty and salt free(Passing through Nefion) one side of nafion(cation exchange) is in contact with...

17 June 2019 3,675 5 View

The reason for increases and decreases of mainlobes and sidelobes in non-rectangular windowing?

I'm interested to find out the reason for increases of mainlobe's width and decreases of sidelobe's amplitude in windowing IIR filters with non-rectangular windows,like Bartlett,Hamming,... .

31 December 2018 5,615 8 View

How can I solve the problem in cloning a PCR amplified gene into a vector?

06 September 2014 899 20 View

Why did I get band for RNA after PCR?

I did cDNA synthesis after RNA extraction including a negative control containing RNA and water with no RT. I used all my samples for regular PCR While I included a negative control containing...

27 February 2014 8,769 14 View

What the cycle of heat treatment for shafts or spools in an engine?

Thanks.

03 February 2014 9,060 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View