Hi there, I've been using the EVOS Auto II microscope for some time now and usually I will save the transmitted light/bright field image and the images from fluorescent channels as separate images, as well as combined into a composite image. Unfortunately, the settings had been changed since I last used the microscope without me realising, and the only images I have saved are the composite images which have the fluorescent channels and transmitted light all in the same image (so the fluorescent colours on a white/grey background).
I really need the fluorescent channel images on their own and wondered if there was any way to separate out the original fluorescent channel images using ImageJ/Fiji. When I use the "split channels" option I get the transmitted light image with the fluorescent channel image overlaid on top and this isn't what I want - I want my original fluorescent channel images (i.e. black background with just fluorescence visible). I will be so grateful if anyone can help! Thank you!
Is it possible for you to upload an original image (with meta data)?
@Sathya Srinivasan
Hi Melanie Seaton , ar you familiar with ImageJ(NIH) software? If you have the raw images this software might be able to serve your issue. Also, you can download different extensions to this software which allows you to open images in many microscope types.
Chathura S. Abeywickrama I'm familiar with ImageJ, my issue is that the only image I have is a composite of the transmitted light field and the fluorescent dark fields, rather than the raw images - do you know how I can separate them out?
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