Hello everyone,
I'm working with a lncRNA that has little expression and I'd like to perform a pulldown assay with it, does anybody has any recomendation about the best way to do this? Thanks a lot
I'm trying to perform RACE PCR with the GeneRacer kit (Invitrogen). Since the first PCR I'm getting bands much smaller than the expected size for the 5' reaction. Also, I'm having trouble with the...
10 November 2018 2,425 4 View
I want to define both 5' and 3' ends of a lncRNA using GeneRacer Kit (Invitrogen). I don't know if my transcript has a poli(A) tail and the manual recommend using an oligo(dT) primer to reverse...
06 July 2018 307 4 View
I'm trying to simulate a patient's chromosomal loss in vitro. I wonder if it's possible to do it with CRISPR/Cas9 or with any other technique.
01 February 2018 534 5 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...
23 February 2021 7,842 1 View
I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...
23 February 2021 9,933 1 View
New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...
21 February 2021 3,278 3 View
Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...
21 February 2021 7,397 1 View
I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...
21 February 2021 6,260 3 View
Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...
21 February 2021 6,633 3 View