Isabela Ichihara de Barros

4 Questions 6 Answers 0 Followers

Questions related from Isabela Ichihara de Barros

Any tips for RACE PCR?

I'm trying to perform RACE PCR with the GeneRacer kit (Invitrogen). Since the first PCR I'm getting bands much smaller than the expected size for the 5' reaction. Also, I'm having trouble with the...

11 November 2018 2,465 4 View

RACE: I don't know if my RNA is polyadenilated, is the RNA oligo from the Invitrogen kit enough for oligo(dT) primer to anneal in RT reaction?

I want to define both 5' and 3' ends of a lncRNA using GeneRacer Kit (Invitrogen). I don't know if my transcript has a poli(A) tail and the manual recommend using an oligo(dT) primer to reverse...

07 July 2018 346 4 View

Any tips for a low expressed lncRNA pulldown assay?

Hello everyone, I'm working with a lncRNA that has little expression and I'd like to perform a pulldown assay with it, does anybody has any recomendation about the best way to do this? Thanks a lot

07 July 2018 6,211 0 View

Is it possible to use the CRISPR/Cas9 system to delete big genomic regions (like 100 kb)?

I'm trying to simulate a patient's chromosomal loss in vitro. I wonder if it's possible to do it with CRISPR/Cas9 or with any other technique.

02 February 2018 570 5 View