We are trying to find the copy number of transgenes in a Eucalyptus plant using DIG labelled probe (700 bp) and color detection. We are using Roche labeling and Detection Kit. However we are getting a blank blot. Any suggestions in this regard from those who are using such system for the southern blotting?
Kiran Kumar
HI Thomas,
I tried with alkaline transfer but transfer was less compare to Salts transfer. Do we need process the before transfer? Can you give any specific protocol for Alkaline transfer.
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