i want to calculate spectral overlapping of fluorescent proteins for FRET. please suggest a suitable software. i have tried some like BD and life technologies but they do not have spectra for all fluorescent proteins e.g., mNeongreen.
thanks Daniel :)
Hi,
In addition to SpectraViewer from lifetechnologies I actively used:
1. Omegafilters
http://www.omegafilters.com/curvomatic/
2. Chroma Technology tool (not online anymore but they have mobile app).
3. Fluorescence Dye and Filter Database by Zeiss:
https://www.micro-shop.zeiss.com/?s=173513366ccbbfd&l=en&p=us&f=f&a=d
Actually mNeongreen is presented there.
BTW, another useful database:
http://www.fluorophores.tugraz.at/substance/
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