Pardha Saradhi Attuluri

5 Questions 6 Answers 0 Followers

Questions related from Pardha Saradhi Attuluri

How can I adjust the pH of organic solvents?

Hi, I want to change pH of an organic solvent 'dibenzyl ether'. Being it is water immiscible NaOH/KOH does't work. is their any other way to adjust pH of this solvent? Thanks :)

08 August 2015 9,271 2 View

Why do I detect no insert after cloning into pENTR D TOPO?

lenght of my inserts vary from 450 to 1.5 kb. all amplification primers have CACC at 5' of forward primer. after topo reaction(followed as instructed in manual) got colonies. when i did colony PCR...

07 July 2015 6,893 7 View

How can I make 2x or 3x fluorescent gene constructs?

I would like to make 3x tagRFP for increasing brightness of target protein. Can you please suggest different methods? Thanks:).

05 May 2015 3,065 2 View

What controls should I use for quantitative imaging of thick tissue samples?

i want to image 70-100 micron tissue for ploidy analysis of nuclei in it. what are the controls i need to use for illumination changes in Z plane?. how much accurate results can be by...

04 April 2015 7,074 2 View

Any suggestions on software for spectral overlapping of fluorescent proteins?

i want to calculate spectral overlapping of fluorescent proteins for FRET. please suggest a suitable software. i have tried some like BD and life technologies but they do not have spectra for all...

03 March 2015 2,854 2 View