I am performing absorption study: carbamazepine drug removal using plant extract capped magnetic nanoparticle (MNPs). In that 5, 10, 15, 20 ppm of drug concentration and 50mg of MNPs I am using. now from zero our to 5 hour (per hour) I am taking OD.

from there I am trying to get percentage of removal :control (zero) initial hour reading minus final hour reading, divided by initial value, multiply by hundred. 

also tried with changing pH parameter 3, main in 7 ph

but In every case i found after 2 hour OD value getting increased beyond control value. 

If anybody having any idea regarding please explain me.

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