What is the density of cis 2 decenoic acid?

Hi I'm trying to make a solution with cis-2-decenoic acid with a product of sigma, but this product is liquid, so I need the density to know how to do this solution and this is not present in the...

16 July 2020 9,125 1 View

How to calibrate a Pitot tube for a boundary layer wind tunnel?

Could someone please recommend me how to calibrate a differential pressure sensor for a pitot tube that will be used in a boundary layer wind tunnel, I bought a Pitot tube 160E Dwyer and a...

03 December 2019 933 3 View

Do C:N ratios for cover crops etc refer to total or organic carbon?

I'm interested in determining the carbon to nitrogen ratio of a woody material that may in due course be applied to an agricultural field. I know that ideal ratio is ca 24:1. I think that's total...

25 November 2019 5,060 3 View

I need guidance regarding time series data analysis. Are there specific methods to address randomness?

The challenges of time series

08 August 2019 6,311 3 View

How aspartic acid react with H2S gas to get thiomide aspartic as output?

I need aspartic acid to react with H2S gas and attain thiomide aspartic as output. I have an idea that either H2S gas passing through Aspartic acid powder directly in closed chamber or through...

06 February 2019 5,325 3 View

Would anyone be willing to share a protocol for a transwell migration assay with murine macrophages (towards chemokine in the lower chamber)?

So far I have only performed migration assays with suspension cells and now I am trying to set up the migration assay with murine macrophages, differentiated from bone marrow cells, in a Corning...

06 September 2018 7,524 1 View

What is this amazing creature that I found on my goldenrod the other day?

I'm trying to document the insects, spiders, etc that live in or visit my backyard habitat. The other day this creature (or two of them, maybe in mating postures) showed up in the yard on a stand...

05 August 2018 2,365 4 View

What are some questions the I can include in my International Marketing Project in a Likert Scale Format?

I am currently working on my International Marketing Project which I am given the topic: Barriers to enter into International Marketing or the emerging market; Evidence from the SMEs in Papua...

02 August 2018 213 2 View

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Paediatric population with Diabetes 1 that requires Insuline Injection Dispositives present complication in their skin for many reasons. We are now trying to development a clinical experience...

29 July 2018 5,611 2 View

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I am trying to fuse a fungus protein with mCherry, I have previously use a flexible linker (Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly-Gly) introducing it by PCR, but the problem is that it cause a kind of...

28 January 2018 3,017 2 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

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01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View

How to calculate Z (the number of molecules in a unit cell) for calculating theoretical density?

I am trying to calculate the theoretical density of my ceramics materials. I use the formula below to calculate the theoretical density: Theoretical density = (Molecular weight x No. of molecules...

24 February 2021 3,823 2 View