We have problems finding a marker for plasma cells. CD138 antibodies (which is used in the literature) are not working in our lab. Any other suggestions?
Plasma cells markedly down-regulate surface markers. The classical way to detect/enumerate them is by enzyme-linked immunospot assay (ELISPOT) that detect B cells secreting antigen-specific antibodies.
The species which I'm working with is mouse. I tried costaining with CD27 and Cd138 but again CD138 is not working.
CD138 works fine in the mouse. One thing you must know is that plasma cells are larger than lymphocytes and might be outside your lymphocyte gating.
https://www.researchgate.net/publication/26888559_Immunoglobulin_aggregation_leading_to_Russell_body_formation_is_prevented_by_the_antibody_light_chain?ev=prf_pub
Article Immunoglobulin aggregation leading to Russell body formation...
I am working on human plasma cells, so not sure whether similar will apply for mouse, but the markers i am using to define plasma cells are: CD27+CD38++ and CD138. Antibodies are from eBioscience and are working fine.
You could try to stain cytoplasmic Ig light chain as a control. Possibly a cytospin will help you to find the plasma cells by immunofluorescence. Use Hoechst or DAPI as a counterstain.
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