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Hello all. I transformed some Agrobacterium competent cells by electroporation and performed colony PCR to see the positive transformants. However, I performed the glycerol stock after 10 days of...
26 May 2024 9,304 3 View
Hello all. I purified some plasmids after doing precultures of strains from a 2-month old plate in -4 degrees. The PCR results were fine and I used theses plasmids to transform Agrobacterium...
26 April 2024 8,440 3 View
On the use of solar energy in homes.
07 April 2024 9,465 2 View
I'm looking for variables like solar irradiation, wind speed, humidity, temperature and precipitation
06 April 2024 1,926 2 View
I have trouble solubilizing the analytical standard of dibenzo[a,h]anthracene (a polycyclic aromatic hydrocarbon purchased from Sigma-Aldrich) to prepare a stock solution. I have already tried...
01 April 2024 5,768 3 View
Hi everyone, I was wondering if someone has a protocol for using MitoSOX in mitochondrial-enriched extracts. MitoSOX can migrate to the nucleus and generate an inaccurate response about...
24 March 2024 466 0 View
I have prepared a liquid medium for fermentation with kraft lignin in water, but the lignin was not completely dissolved. After fermentation, I am wondering about the best way to filter out the...
04 January 2024 7,479 1 View
I am trying to see a quencher (BHQ)tagged synthetic DNA oligonucleotide on an agarose gel...I used EtBr for the same...I was able to visualize the strands that I have (not tagged by BHQ), but I...
20 December 2023 2,128 0 View
I've tried to use Ripa buffer containing 0.1% SDS + 10% of proteinase inhibitors combined with 3 cycles of freeze/thaw (Dry ice with isopropanol/Dry bath 70ºC), followed by 16.000rcf for 10...
17 December 2023 3,030 1 View
Ineed a recipe.
13 December 2023 9,782 0 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
I am reaching out to seek your valuable advice and recommendations regarding the best software tools to use for this research. Specifically, I am looking for software with a user-friendly...
22 July 2024 3,794 1 View
Hey all, I need help testing for multivariate outliers using STATA for my master thesis. The literature recommends the Minimum Covariance Determinant (MCD) (Verardi & Dehon, 2010). I found the...
22 July 2024 8,821 2 View
Dear Researchers, My question is associated with the "MDCI" method in Orca. Please let me clarify my question using a simple example: Suppose we are going to perform CBS extrapolation using "!...
21 July 2024 1,632 0 View
What type of experimental design do l use and what statistical tools do l use for the hypothesis. What is the position of control group in the analysis?. Thanks
18 July 2024 3,581 3 View
im studing gaussian 16 with reading paper about I-131 Metaiodobenzylguanidine in the paper "In a similar vein, nuclear magnetic resonance shielding values were investigated using the widely...
16 July 2024 6,040 4 View
I am a 3rd year Computer Science student currently writing our Bachelor's thesis about finding diverse k-shortest paths in pedestrian networks. We have chosen 3 local areas as our proposed...
15 July 2024 4,289 0 View
Hi all, Do you know how to plot this figure? I found many of them in Nature journals but have not figured out how to make them. Which software/tool do you suggest? Thank you very much.
15 July 2024 8,384 2 View