14 June 2021 6 3K Report

Protein extraction, wastewater treatment, anaerobic bioreactor

More Stephanie Greige's questions See All
Are primary & secondary antibodies needed for flow cytometry to detect EGFP transfected cells?

Hello, I am new to designing flow cytometry experiments. I have some cells transfected with EGFP using mRNA and want to quantify its percentage expressed. I also wish to differentiate between...

17 May 2024 8,527 2 View

Where to find cloud cover estimation during the night for Southern Africa?

Hello RG community, I am looking for remote sensing data on cloud cover estimation on a daily basis but during nighttime over Namibia /Southeast Africa. The ideal spatial resolution would be 1km...

31 March 2024 298 2 View

Does anyone try renaturating inclusion body with copper sulfate?

I am currently doing isolation of protein from inclusion bodies. I solubilize the protein with 8M guanidine hydrochloride (Simpson, CSHL, 2010). I this step I saw no pellet anymore in my sample....

17 March 2024 8,573 0 View

How to form the immune complex using human IgG?

How to form the immune complex using human IgG?

16 November 2023 3,811 1 View

I want to study the male reproductive system for two different seasons. Please guide me how much should be my simple size be?

I want to study the male reproductive system for two different seasons to obtain histology, histometery, morphology and morphometery. Please guide me how much should be my simple size. What would...

22 May 2023 7,177 5 View

Pretest-Posttest Nonequivalent Groups Design: ANCOVA or Change-Score Analysis or Something Else?

I am working on my dissertation, and I am stuck on the type test I should run on my data. Research Design-I have a control and a treatment group where a vocabulary intervention was given to the...

02 April 2023 1,263 4 View

Help with shRNA gene silencing?

I am trying to do a shRNA gene silencing for an esophageal cell line. Does anyone have any specific protocol to suggest?

27 March 2023 6,675 3 View

Can you modify BSA with NHS-PEG4-azide?

I performed a reaction to modify BSA through esterification, but it didn't seem to work. Most of the papers that I've found that attach azide to BSA seem to use maleimide. Is there a reason why...

09 March 2023 4,971 2 View

Policies adopted by state sector to meet the objectives as welfare of citizens?

policies initiatives during past few years

08 March 2023 5,540 2 View

Interpretation of Data ?

How do we as scientists interpret data to provide the most explicit explanation for our research? I am aware that we can present the limitations of our study which can defer our...

15 February 2023 2,867 1 View

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Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

What roles do microorganisms play in Earth's ecosystems, & why is their role so important and role of microorganisms in secondary wastewatertreatment?

What roles do microorganisms play in Earth's ecosystems, and why is their role so important and role of microorganisms in secondary wastewater treatment?

23 July 2024 5,032 3 View

From different feedstocks based Biogas digesters what would be the standard NPK - Nutrient contents of the digestate, and how consistent is it?

One has to list down the details and understand that different feedstocks and the biogas digesters working on different feedstocks show different nutrient content value: But when it comes to...

18 July 2024 9,907 12 View

How can the economic value of digestate management, utilization, and regulation, can spark interesting discussions and explorations?

How can the economic value of digestate management, utilization, and regulation, can spark interesting discussions and explorations? so one can list down the details and we can discuss in length...

17 July 2024 3,255 4 View

Environmental Impact of the Organic Manure Production Unit?

Does anyone have any case studies on the Environmental Impact of Organic Manure Production Unit? Environmental Impact of a Organic Manure Production Unit Facility is what I studied but...

16 July 2024 5,400 6 View

What could be the possible reason that sintering of polycrystalline diamond fabricated with tape casting has suddenly started failing?

I make polycrystalline diamond with tape casting followed by HPHT sintering. When i sintered the samples up till May, the sintering was good. When i sintered the samples in June and July at the...

16 July 2024 3,212 5 View

Can I use candle-jar technique to lactic acid bacteria?

I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria

15 July 2024 8,890 2 View

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