Any reference steps for maximun/minimum Ct values-based relative quantification of bacteria ?

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After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

How to use -dhdl flag during mdrun with gromacs?

I am trying to use the gmx energy model to make free energy calculations upon binding of a ligand to a receptor. To do this I am invoking the energy module and adding the -odh flag: gmx energy -f...

09 November 2020 1,631 3 View

Preparation of mouse thymus for Flow Cytometry?

Hi, I'm trying to develop a protocol for prepping thymus tissues in mice. We will be using NSG mice in our lab and the goal is to get good consistent single cell suspensions to stain for FACS....

26 October 2020 10,006 3 View

Issues generating hbmap.xpm file from gromacs hbond module, using -xpm flag?

I am currently trying to monitor the changes that a ligand elicits in the hydrogen bonding pattern of a protein during binding. I can easily see with the -num flag that the number of internal...

18 August 2020 7,973 1 View

How to publish my old paper in an open journal?

Dear Research Gate Community, I'm finally able to publish my dissertation paper and I'm keen to find the right place to publish it. My old university has just given me permission to publish it...

13 July 2020 1,938 3 View

Recruitment of Children - Ages 5 - 12 year olds - best methods?

Hi All, I am running a study which requires two visits for children to complete an EEG (Brain scan) and an eye tracking task. We are currently working a recruitment plan for after the Covid-19...

30 March 2020 8,737 3 View

Which methods do academic writing and literacy facilitators apply to incorporate students' primary discourses into learning experiences?

Greetings Colleagues Primary discourses are those ways of using language, literacy and multiple modes of communicating as acquired in our homes, among our families and in our initial schooling...

28 December 2019 6,472 5 View

Is social media a good platform that will bring change in elections?

South African elections have ushered in a new thinking on the role of social media in influencing elections. The EFF had the highest number of young people who where on social media. ( 6 million)...

12 May 2019 4,031 6 View

How much solvent (ethanol) would I need to extract capsaicin (and other capsaicinoids) from siling haba?

Siling haba is a variation of Capsicum annuum var longum. The capsaicin extracted will be tested against aphids to test its insecticidal properties. Someone suggested that I should use 5ml/g or 2g...

12 February 2019 1,373 3 View

FMRI power analysis for RSA (representational similarity analysis)?

Dear community, I'm looking into ways how to do an a-priori power analysis for an fMRI experiment where the main analysis will be a representational similarity analysis (RSA). The experiment...

06 February 2019 1,338 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

How to get rid of somebody pretending to be the author of a publication?

One of the publication I co-authored has the author's name in abbreviation. Then, somebody whose nothing to do with that paper acquired it as her work. To whom in this RG shall I ask for a hand,...

27 February 2021 4,206 4 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Can I put a figure that was drawn by Bio-Render in publication paper?

I want to publish paper in journal but I have figures which have drawn with bio-renders, can I put them in the paper or which other software I can use to draw them?

27 February 2021 8,179 1 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Why is My firend's profile not visible?

Hello, I created a profile for my friend on google scholar. I have added a verified email address. I marked the profile as public. Formerly his profile was visible. However, now, When I type his...

24 February 2021 8,680 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View