Does anybody have any experience with primary cancer stem cell culture? I want to isolate and to culture cancer stem cell from human skin cancer tissue. Do you have any protocol?
Hi everyone, I have a question in the case of, codons and regions in the whole DNA. I wanted to design a pair of primers that include 167, 198 and 200 codons. But I couldn't find them in the whole...
29 November 2022 3,518 1 View
I have done a lot of PCR to identify a mutation in beta-tubulin. I have two sets of primers, one is for mutant and one is for wild type. I did nearly 100 PCR but I have a problem getting a good...
27 November 2022 5,773 6 View
Looking for arguments, facts or assumptions that would sustain or completely dismiss this hypothesis.
27 June 2022 5,620 1 View
I'm doing a PCR for detection of Mutation on position 167 in beta tubulin. It is a Nested PCR which a second reaction was performed using the primers Fs + R2 (141 bp) and F1 + Rr (212 bp). The Fs...
18 June 2022 5,712 3 View
Hi everyone I have made a Stock Solution of my primer 100 uM, and I also have a Working Solution of 10 uM. can I convert my working solution 10uM to 5uM directly?! if I want to make a 5uM working...
20 January 2022 5,510 4 View
Hi everyone I tried to do PCR, and I expected to get a band in 121, but is my third PCR that I have gotten 400bp band. I Checked my primer and all of the other parameters. it should be mentioned...
08 December 2021 2,102 10 View
Hello everyone, I have all the hookworm ITS-2 sequences and I want to check where the primers are present and the size of the amplicon with an alignment software (NCBI Blast). what should I do? I...
15 October 2021 1,536 6 View
i want to confirm my finding regarding Ancylostoma Caninum eggs by molecular studies, which is ``ITS-2 amplification``. but the number of eggs in my samples is not to much and i cant get any band...
29 August 2021 965 11 View
I want to find scattering fields and RCS for infinite cilynder in CST can anyone help me
01 June 2021 6,905 2 View
Dear researchers, I plan to do a duplex qPCR with two primers and each primer has a probe. First one uses Cy5 as fluorophore and BHQ-1 as quencher and the second one uses FAM as fluorophore and...
11 February 2020 5,756 2 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View