Can I replace a flurophor with another one for qPCR probes?

Dear researchers, I plan to do a duplex qPCR with two primers and each primer has a probe. First one uses Cy5 as fluorophore and BHQ-1 as quencher and the second one uses FAM as fluorophore and...

11 February 2020 5,666 2 View

Does anybody knows anything about this instrument " the nemescope"?

http://rexresearch.com/nemes/1nemes.htm is this real...

08 January 2020 6,056 2 View

How can I extract S-layer proteins?

Dear researchers, I need some advice from more experienced people, please. I have a bacterial strain which is producing both S-layer proteins and a bacteriocin with similar MW. How can I...

05 November 2019 4,059 3 View

Dear network users, Is it possible to simulate in ANSYS shear testing of thermal sprayed layers? Shall I use INTER 202?

I would like to make a stress analysis at the interface of thermal-sprayed layer and the base material during the shear testing.

17 May 2019 7,355 2 View

Can you recommend me some articles that dealing with the electronic transitions of toluene?

Hello! I'm working on assigning absorption bands to UV-vis spectra of unknown matrix. I think some are due to the presence of toluene, but I can't find any article that describes the electronic...

19 March 2019 8,119 3 View

Should I use a chi-square test? I want to compare the level of a compound produced by different yeasts.

15 August 2017 4,955 5 View

Can anyone indicate some references about the applications of driftless control affine systems in economy?

Can anyone indicate some references about the applications of driftless control affine systems in economy?

13 October 2016 9,794 2 View

How do I build up multiple layers of V2O5?

I'm trying to produce samples with 6-7 spin-coated layers of V2O5. The first layer goes on great but when I apply the second layer it 'pulls' the first layer off. Slides are cleaned using piranha...

10 April 2016 8,956 2 View

Can one use the 'true capacitance' extracted from a CPE to subtract capacitive currents from a CV in order to perform peak analysis?

I am trying to do peak analysis on anodic biofilms in microbial fuel cells. To achieve this I am running CVs at different scan rates and then potentiostatic EIS. Because I noticed EIS behaviour...

10 September 2015 4,947 1 View

Can you please recommend any companies selling good quality Graphene Oxide, Graphite Oxide or Graphene (CVD/ Epitaxy)?

I am looking for an alternative to "home made" graphene/graphite oxide and into prices for graphene films. Thanks!

29 November 2014 5,235 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View