Дорогой доктор Рональд Мальдонадо Родригес. Спасибо за интерес к нашему проекту. К сожалению, из-за прекращения финансирования в нашей стране это и другие работы были прекращены. Мы ищем спонсоров для решения этой проблемы .... Основным направлением наших работ является разработка оптических методов диагностики в широком диапазоне длин волн 0,4-14 мкм на месте и дистанционное зондирование с целью точного измерения стрессовых реакций растений в системе почвенных растений.  В последних исследованиях мы используем активный оптический красный / IRED-тестер для устойчивого сельского хозяйства и испытания специальных бактериальных препаратов.                   С уважением, Владимир Сурин

Dear Dr. Ronald Maldonado Rodriguez. I am sorry for the Russian version. Thank you for your interest in our project. Unfortunately, due to the termination of funding in our country this and other work has been terminated. We are looking for sponsors in order to solve this problem ....

The main direction of our work is the development of optical diagnostic methods in a wide range of wavelengths of 0.4 -14 mkm in situ and remote sensing with a view to the precision measurement of stress reactions of plants in the soil-plants system.

In recent studies we use active optical red/Ired tester for sustainable agriculture and  testing of  bacterial preparations in  Life Cycle of crops. Sincerely, Vladimir Surin

Dear Dr. Vladimir Georgievich Surin

I am so sorry to hear about the termination of your Research Project(s).

I do hope you may be able to find sponsors as quick as possible to continue with your research work.

It seems that since the 2008 crisis, the problem with funds for science is global, emerging everywhere even in most rich and developed countres and it's getting worse. Right now, we can speak of "darwinism in research".

From your answer, I assume that you are applying only absorption/reflectance red/Infrared remote sensing optical methods and that you have (not yet) included measuring spectral Chl a Fluorescence (static or/and dynamic), isn't?

I believe, you could create more interest in potential sponsors (private sector, national agencies, european projects) if you could propose adding optical methods for determining leaf tissue biochemistry, some remote sensing of vegetation by using solar-induced Chlorophyll fluorescence (e.g. Fraunhofer lines) combined with math models of crop radiative transfer functions, some chemometrics and artificial neural networks methods for signal analysis and classiffication. See also http://fluorflight.com/

National agencies related to forest fire prevention should be interested in remote sense applications; forest and vegetation water content is directly related to this serious problem.

Dear Vlado, just a couple of questions for you:

1) I guess, you are doing direct spectral reflectance measuring experiments to calibrate airborne remote sensing experiments. And I guess, maybe you have access to hyperspectral data... Are you aware of the method developed by Raymond F. Kokaly et Roger N. Clark about analysing remote sensing AVIRIS hyperspectral data for quantifying leaf biochemistry (e.g. Nitrogen content) in remote sensed vegetation?

Kokaly have two beautiful papers about this interesting method:

International Journal of Applied Earth Observation and Geoinformation

Volume 43, December 2015, Pages 55-83

Plant phenolics and absorption features in vegetation reflectance spectra near 1.66 μm

Raymond F. Kokaly & Andrew K.Skidmoreb.

https://doi.org/10.1016/j.jag.2015.01.010

Remote Sensing of Environment, Volume 67, Issue 3, March 1999, Pages 267-287

Spectroscopic Determination of Leaf Biochemistry Using Band-Depth Analysis of Absorption Features and Stepwise Multiple Linear Regression

Raymond F.Kokaly & Roger N.Clark

https://doi.org/10.1016/S0034-4257(98)00084-4

2) Recently, John Couture et al. have developed an interesting method for quantifying living vegetation biochemistry (e.g. relevant plant secondary metabolites) related to Kokaly's works. I guess, by extending the analytics of current spectral methods, reflectance spectroscopy among them, they could be used to evaluate the impact of air pollution in crops and forest, what do you think about it?

3) Do you have a web site related to your projects?

Kind regards,

Ronald M. Rodriguez

The Fluoromatics Lab

Geneva, Switzerland

www.fluoromatics.com

Sehr geehrter Herr Kollege,  Meiner Meinung nach ist das Thema  und das Gebiet ueber  die Wirkung der schwer Metalle auf Chl a Fluorescence  sehr interessant  und dabei nur provisorisch unretsucht. Mittels PAM-Method koennen Sie erstaunliche Ergebnisse bekommen.   Mit freundlichen Gruessen aus St.-Petersburg . V. Saakov

Thank you Prof. Saavov,

I do highly appreciate sharing your comments and experience on PAM measurements with us. They for sure are a great technique in plant research.

Have you ever coupled PAM measurements to spectral methods like reflectance spectroscopy in order to compare results from both techniques?

Best regards,

Ronald M. Rodriguez

The Fluoromatics Lab

Geneva, Switzerland

Original message "Dear Colleague, In my opinion the topic and the area is very interesting about the effect of heavy metals on Chl a fluorescence and is only provisional. Using PAM method, you can get amazing results. Yours sincerely, from St.-Petersburg. V. Saakov"

Dear Ronald M. Rodriguez. Thank you for the useful information. But we have another problems now - crisis and sanctions. We  wish success to you colleagues. Best regard. Vladimir Surin/

Hello Dr. Ronald ...I am retired but style alive. Yes, there are some grasses, Brachypodium sp. , cariologically related with the cereals for human consumption (wheet , barley , corn). Would be very interesting to research their performance on heavy metal soils.

Buenos días mi querido Alberto! I am very happy to have news from you.

Yes, we can connect by e-mail to discuss how we can proceed for doing that kind of research, grasses growing in heavy metal polluted soils. My e-mail is [email protected]

Un fuerte abrazo!

Dear colleagues.

In our experience (over 20 years) one of the most effective and economical way to evaluate the impact of heavy metals on plants in their life cycle in situ is a photometric method using active optical tester. To obtain the results might be used a two channel sensor . This instrument allows to made measurements under any weather conditions at any time with high accuracy about 1%.

Procedures of measurements developed by us which permits to reveal and to identify a different phases of stress reactions in plants and define their borders, expressed in photometric units. This will reduce the volume of the control chemical analyses. Using these analyses can be conducted evaluating the toxicity of different elements at different stress phases and identified antagonists and synergists in plants, etc. ... Techniques were tested with different sensors, on sites with different levels of pollution from low-contrast plots on ore deposit structures to off toxically waste sites.

On their basis of satellite data processing algorithm was developed. As a result of using Landsat-TM system on the Kola Peninsula was revealed natural geochemical anomaly caused by heavy metals in conditions of interference anthropogenic emissions from the Monchegorsk metallurgical plant.

If field trials will be conducted with Brachypodium SP it is very important to compare the information content of the data which obtained with luminescent and photometric methods in situ. There is but little a quality information about it now!

We are ready to take part in this interesting experiment.

Sincerely, Dr. Vladimir Surin