It depends on your target molecule(s), expected concentration, and sample matrix!

Hi Sunil: What kind and size of compound and molecules you want to purify? 

Hi Sunil

Some references in attachment 1

http://cactus.dixie.edu/smblack/chemlabs/Separating_Compounds_by_Column_Chromatography.pdf

2

https://courses.chem.psu.edu/chem36/Experiments/PDF's_for_techniques/CC.pdf

3

https://chem.rochester.edu/~nvd/pages/troubleshooting/_flash_chromatography.php

4

https://www.google.com/search?q=Column+Chromatography+procedure%2Fprotocol+%3F+I+want+to+purify+my+compounds#q=Column+Chromatography+procedure/protocol+to+purify+my+compounds

5

https://ocw.mit.edu/courses/chemistry/5-301-chemistry-laboratory-techniques-january-iap-2012/labs/MIT5_301IAP12_FlashHandout.pdf

6

https://www.unesco.org/science/doc/Organi_chem_220709_FINAL.pdf

7

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3218439/

8

https://www.ncbi.nlm.nih.gov/books/NBK21589/

9

https://www.reachdevices.com/SetUpColumn.html

10

http://dspace.ewubd.edu/bitstream/handle/123456789/24/Mahmudul%20Hasan%20Chowdhury.pdf?sequence=1

Thank you sir for providing useful information