Jelly fish DNA isolation is difficult for me. I followed Qiagen Dneasy  Blood and Tissue kit. DNA from Cyanea species Obtained from that kit. Other species DNA could not get from that kit.I used the primers LCO1490, HC02198(Folmer et al. 1994) with annealing temperature 45 for all species. But could not obtain a good result. I also use LCOjf, HCO2198(Folmer et al. 1994)With annealing temperature 50 degrees. I am repeating sample several times with different primers but I can't standardize DNA isolation till now . Please anyone help me to clarify C- Tab phenol Chloroform methods and  reagent  preparation for jellyfish DNA isolation ?