We are working on Carnosine (alanyl-histidine), which are using for encapsulation in nanocarriers. We possess pure carnosine which we plan also to use to make a standard curve. I am trying to find a method to determine carnosine concentration.
- Bradford, even though sensitive to histidine is unable to measure dipeptide.
- Since the peptide is mixed with solvent (chloroform), the interference of the solvent is an issue.
- the lack of aromatic groups prevent also the use of 280nm reading.
Given the above, I would appreciate if you could suggest an approach to determine the concentration.
Thank you
Hichem Moulahoum
Hello everyone,
After some research and inquiries with others, we found out that a direct reading at 205 nm (UV) is the most suitable way to solve the issue. The wave length is specific to the peptide bond and because the carnosine used is pure, we decided to use this method.
The presence of organic solvents were eliminated using the according blank.
Any other suggestions are also welcome!
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