Indian Institute of Science, Bangalore, University of Agricultural Sciences, Bangalore are equipped with this facility.

Check this link maybe helpful

https://m.indiamart.com/impcat/hplc-analysis.html

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yes you can use this protocol

for lysine analysis

Samples of feed and meat were digested in acid and alkaline medium to the complete hydrolysis of the protein fraction. Briefly, 100 mg of each sample were digested with 3 ml of 6 N HCl at 200 0C in heated oven for 24 hours after sealing tubes with nitrogen gas to prevent oxidation. The digested samples were filtered with Whatman No. 6 and the filtrates were evaporated at 100 0C water bath for removing the chlorine gas. Hydrolyzed protein was completely dry with nitrogen gas and re-constituted with 200μl (0.2 ml) of 0.1 N HCl.

Following hydrolysis, centrifuged for 10 minutes at 4,000 RPM and then supernatant was taken and diluted with 50 folds with water (milliq water). Then the final acid and alkaline hydrolyzates were filtered (0.2 µm) and inject into UHPLC system using MPA/OPA/FMOC derivatization protocol. Mercaptopropionic acid (MPA) used as catalyst, o-Phthaldialdehyde (OPA) and Fluorenylmethyl chloroformate (FMOC) used as reagents for primary and secondary amines derivetization, respectively.

UHPLC instrumentation and analytical procedure: Amino acid analysis was conducted with the Shimadzu UHPLC system (Shimadzu, Columbia, MD). Derivetaization was taken automatically by the instrument using o-Phthaldialdehyde (OPA) for all primary amino acids and Fluorenylmethyl chloroformate (FMOC) for secondary amino acids (Proline and Hydroxyproline). The UHPLC system consisted of a binary pumping system: pump A (LC-10AD VP) and pump B (LC-10AT VP), a degasser (DGU-14A), an Autosampler (SIL-20AC HT), column heater (Brinkmann, CH-30) and Fluorescence detector and system controller (CBM-20A). Mobile phase A was a mixture of Sodium hydrogen phosphate (Na2HPO4), hydrated sodium borate (Na2B4O7) and Sodium azide (NaN3) while mobile phase B was acetonitrile/methanol/water (45/45/10 v/v/v).

The separation was obtained at a flow rate of 2 ml/min with a gradient program that 0.01 min (1% B), 7.4 min (40% B), 10 min (45% B), 10.1(100% B). Then washing at 100% B and calibration at 0% B was performed in a total analysis time of 12.1 minutes (Carl, 2015). In order to quantify amino acids, the mix standard was used from Asparagine, Alanine, Arginine, Aspartic acid, Cystine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Serine, Tyrosine, Valine, Proline, Tryptophan, Cysteine, Norleucine and Hydroxylproline were prepared and used for easy identification of peaks in the mix as well as their individual amino acid standards. Before real sample analysis, the UHPLC was tested for linearity, precision and limit of quantification (LOQ), selectivity and resolution by spiking amino acid standards (Kim, et al., 2013).