I am wondering whether someone has idea of GFP cell surface display? how strong the fluorescence signal under microscope? I am thinking of promoter GAD-Aga2-GFP in yeast and promoter?- lpp-ompa-EGFP in bacteria.
Hello, Stringent microbial cell barriers limit the application of many substances in research and therapeutics. Carrier peptides that penetrate or translocate across cell membranes may help overcome this problem. The known way is peptide-mediated delivery of green fluorescent protein into yeasts and bacteria by the attached method.
Good luck !
Thank you Karen A. Darbinyan. Interesting paper although my question is opposite to the paper----delivery of endogenously expressed GFP to the surface of microbe cell.
I am particularly interested in finding a photo of GFP expression in cell surface by surface display technique.
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