What is the Timestep definition in the Conservation Element / Solution Element in 3D ?

15 November 2020 1,214 3 View

Does anyone know of tested survey questions or value statements to evaluate consumer ethics for food and agriculture?

I'm looking for tested survey questions or value statement that address topics of food and agricultural ethics and consumer values for food purchasing... is anyone aware of an existing study that...

01 July 2020 301 2 View

Is free cytosolic GFP fluorescence excluded or reduced in the endomembrane system (ER, Golgi, lysosomes)? Why or why not?

As soluble, untagged GFP is not membrane bound and doesn't contain targeting or signal sequences, one might expect it to be synthesized by free ribosomes and not bound ribosomes. That would mean...

08 March 2020 997 3 View

How many base pairs mismatches should i consider to design a specific primer? my templates have 1-2base pairs mismatch only when i aligned them?

i need to design a specific primer for each of my samples. but my samples, when i aligned them, they only have 1-2 bp mismatches, barely 3

17 September 2019 6,650 3 View

Can you recommend a fluorescent ELISA agent?

Using a fluorophore rather than a chromophore for ELISA detection seems like an obvious solution to increasing ELISA sensitivity, but I'm struggling to find work done in this area. Can anyone...

07 August 2019 2,604 5 View

What are the Arch Garch conditions?

20 April 2019 3,704 4 View

What is a best way to immobilize RGD peptide with electrospun nanofiber?

I synthesized the nanofiber via electrospinning but need to find a best way to immobilize RGD peptide (Arg-Gly-Asp) onto naofibers.

03 September 2018 8,448 1 View

How to run longitudinal mediation with multiple (5 waves)?

Hi everyone! I'm doing a PhD in Clinical Psychology and I have some treatment data to analyse. The design is a 2 (condition: self-compassion, cognitive restructuring) x 5 (time: baseline,...

01 August 2018 4,502 3 View

Increased thermopower (Seebeck coefficient) with rising temperature? (p-type semiconductor). Is this the case for any p-type sc? What about n-type?

28 March 2018 4,475 3 View

Why do my western blots look so odd?

The three images attached here are for 3 different proteins probed at the same time. The first one is GAPDH. Does anyone have any idea why (1) Despite reasonable Bradford assay results, GAPDH...

14 January 2018 5,544 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View