Tool for assesing sexual health of women living with HIV?

I would like to assess Sexual health in a group of women of reproductive age group living with HIV. Can anyone kindly recommend a concise but comprehensive and widely accepted tool from which I...

22 August 2018 1,636 2 View

Calculating cross linking density for ionic hydrogel

14 April 2017 2,600 1 View

Can SDS-PAGE Electrophoresis be used as a tool for assessing extent of protein denaturing and can this affect the weight of the product band formed?

Processing of protein.

05 February 2015 639 8 View

What influences inter-day reproducibility in UPLC-MS/MS analysis?

I was analyzing some metabolites in human plasma. While the intraday precision of these metabolites was acceptable ( less than 20%), the interday precision was quite large. So what influences...

13 February 2021 9,183 9 View

Cells not growing in large scale protein expression?

I am using KRX cell. I have grown my overnight cultures at 37 degrees. When I inoculate 1L TB media, my cells do not grow. I have also tried using 25 degree incubation of overnight cultures and...

10 February 2021 3,871 3 View

A good day to you, Great minds. How do I evaporate hexane and Ethyl acetate extract of bacterial metabolite?

I'm really struggling with it. It takes hours on a water bath at 50 degrees

03 February 2021 1,619 3 View

Why some samples don't give any peaks in HPLC when the rest does?

Hello everyone, Last week we ran an HPLC assay with bacterial supernatant samples. The metabolites measured were SCFA and I used a dilution factor of 2 for some samples and 7 for others (because...

22 January 2021 8,667 6 View

How to calculate the CFU/ml for Mycobacterium tuberculosis growing in 7H9 broth?

I am running an experiment in which I need to calculate the CFU/ml of a Mycobacterium tuberculosis growing in 7H9 broth. Is there a reliable method to calculate cfu/ml for MTB since a culture of...

22 January 2021 10,027 2 View

How to revive bacteria from glycerol stock?

19 January 2021 7,025 4 View

How long I can store plasma in -80 oC before doing Growth differentiation factor-15 assay ? , Is it different when I stored plasma at -20 or -70 ?

I want to do GDF-15 assay on multidrug resistant tuberculosis patient

29 December 2020 6,764 1 View

What are the invivo screening methods for anti TB drug?

what are the invivo screening methods for anti TB drug?

25 December 2020 722 1 View

What is the role of pO2 and pCO2 in cell culture?

Using a Nova bioprofile (https://www.fannin.eu/wp-content/uploads/2018/07/BioProfile-Flex.pdf) to sample my CHO culture daily I get various metabolite results and also pO2 and pCO2 results. I am...

13 December 2020 7,998 1 View

Iam looking for articles on mycobacterium tuberculosis?

Hellow my fellows Iam working on Real time PCR technique for detection the gene who is responsiable on Tuberculosis as well as the AFB stain so i hope that any one have worked on the same project...

02 December 2020 2,123 3 View