Hello,
I am trying severalethods of analysis for several hydrophilic compounds and I seem to not have good signal for Ofloxacin.
I use ammonium acetate 5mM at pH 3.5 and ACN with 5 percent IPA in a 20 min gradient.
The Ofloxacin out of the 4 pharmas is the worst.
I used IPA and 5mM of ammonium acetate because it kept screwing the ESI and polymerizing making it no signal after 20 samples.
Any good method available?
Thanks!
suggest You check the literature for cyprofloxacin - it probably has the same troubles.
Polymerising is probably due to Your Acetonitrile. Which instrument are You using?
Yes it is polymerizing due to ACN, I have increased the amount of IPA to 5% and decreased the amount of ammonium acetate to 5mM and the electrospray is fine now.
Ciprofloxacin also has some trouble, I was just wondering really why these compounds in particular are so difficult to detect. I know that in reverse phase they are quite easy to see in a C18 column that is why I was a little bit confused to why I can't see them in Hilic with the LC MS QTOF
You need to run Ciproflox with a bit special care we run it in a multimethod and it performs really bad. Thats what everybody experiences. in selected cyproflox methods it can be done in a good stable way
Thank you for this answer. I was wondering why it did not show up lik trimethoprim or caffeine and I thought acidifying the mobile phase would solve the problem but apparently not. Tricky compound
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