WGBS deeper or more biological replicates?

Hello all! I want to perform WGBS with sheep DNA samples. Due to funding restrictions I have to choose between sequencing deep or biological replications. What would it better, to sequence 3...

23 January 2018 5,446 2 View

What alternative product do you use to prepare HL5 culture medium?

Thiotone E Peptone (ref 212302) has been discontinued. Could you recommend an alternative product? Thank you

05 November 2015 3,517 3 View

What HL5 culture medium do you use? Homemade stuff or Formedium medium?

18 July 2015 7,228 1 View

Can a single item measure mediate between 2 latent variable in SEM?

Is it correct to run this model with SEM (structural equation model) X (LATENT variable: 2 dimensions)--> Z (OBSERVED variable: single-item measure)--> Y (LATENT variable: 2 items)

20 November 2014 9,836 6 View

What is the best reference miRNA in qPCR of miRNA when working with human cancer cell lines?

I am having some trouble with RNU6B as endogenous control for several ovarian cancer cell lines. Could you recommend me other stable reference miRNAs that have worked in your experimental approaches?

20 June 2013 890 19 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

How to handle studies which report perfect effect sizes in a meta-analysis using Pearson's r?

I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...

24 February 2021 2,265 3 View

How to read a flow cytometry graph detailing cell cycle?

Hello everyone, When determining cells in different stages of the cell cycle, cells are stain for Brdu and Propidium Iodide. I understand how PI can be used to discriminate between G1 and S/G2...

18 February 2021 3,920 3 View

What is the original citation for the commonly used activity factors for converting basal metabolic rate to total caloric need?

I see the same activity factor (multipliers) in research papers and websites, but I cannot find where they come from. Does anyone know where these originated? I am looking for an original...

18 February 2021 7,635 2 View

How do you interpret multiple meta-regression in STATA?

Please could someone recommend a resource that explains performing meta-regression with multiple moderators. I have read the STATA META ANALYSIS REFERENCE MANUAL, but this only gives examples of...

17 February 2021 7,376 1 View

How to report Fishers Exact test from SPSS in a report properly?

I am wondering how do I write the data I have collected from my fishers exact test into words so that it backs up my work? the exact sig.(2-sided) was 0.033, meaning it is significant because its...

17 February 2021 3,441 7 View

Aspen Plus Error: Unknown or undefined bead number?

Hello, in my Aspen Plus Simulation (see attachment) there are two heat exchangers. One HEX1 (oil+gas) ist fed by hot gas which ist coming out of a burner. The other HEX2 (gas+gas) shall work as a...

14 February 2021 8,096 2 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

Moderation test of reflective construct in AMOS?

Hi dear researchers, I need your guidance on the moderation test of a reflective construct (i.e., Uncertainty avoidance with three items) with high and low UA. I run the moderation test with the...

08 February 2021 370 7 View