Any good antibody for myelin other than MBP ?

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Any suggestions for good axonal regeneration marker other than Gap 43 antibody using immunohistochemistry?

I have spinal cord cryosections where I would like to check the axonal regeneration after the spinal cord injury. We tried GAP 43 which seems to be not specific for regeneration in our case. Now...

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Does anyone have experience in retrograde labeling PFA fixed spinal cord sections with carbocyanine tracers or any other tracers?

I would like to label regenerating axons after spinal cord lesions. Can anyone recommend how to label fixed tissue sections with lipophilic tracers?

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Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

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Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

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Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

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Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

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Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

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How does an antibody have full intact antibody + half antibody?

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Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

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RIA. How to measure?

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Anyone know of a good progesterone antibody?

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Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

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Nemat Khan

Would suggest:

For Immuno: Anti-CNPase Antibody

For Histo: Black-Myelin Gold Staining

Malleswari Challagundla

thank you for the response

I will try

BTW, why you opted for looking Ab other than MBP ? Other easier option is Flouromyelin stain from Millipore thats only 20 min staining and works well.