Hello, I am looking for a free image processing/analysis software that can analyze images captured during a crystallization process. I want to be able to measure the sizes (mean) of the crystals/particles during the crystallization process (from nucleation to aggregation). I already have the images captured using iC PVM Mettler toledo V19 microscope.

I tried using ImageJ, but I wasn't getting good results as it shows the sizes were decreasing. Usually, the sizes at nucleation should not be the same after some hours of reaction/aggregation. Clearly, the sizes of the crystals increase in size after some time of aggregation reactions. Any other suggestions on software or even better ways to use ImageJ software to measure micrometer-scale sizes of crystals are welcome.

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