IL1a is a consitutive cytoplasmic protein that could be secreted upon pyroptosis, unlike IL1b, it does not require cleavage for being biologically active.
So I was wondering if anyone has experience quantifying this protein in tissue (either pro-IL1a or IL1a) by quantitative methods like ELISA?
Hi, Georgina Lopez- Cortes
This is a good idea, but be careful to choose an antibody that can distinguish between pro-IL1α (full length) and mature IL1α (partial sequence may be missing at the N-terminus) to avoid cross-reaction.
Our laboratory has tested IL1α secreted into the interstitial space and outside the cell. You can collect tissue culture supernatant (such as organ culture system) or collect perfusion fluid after perfusing the tissue with PBS, centrifuge at 4℃ to remove impurities, and then directly test.
If you are still working on the topic of pyroptosis, you can take a look at the poster of pyroptosis pathway we provided, I hope it will be helpful to you.
Poster Programmed Cell Death Pathways-Autophagy, Pyroptosis, Ferrop...
The answer to this question comes from MedChemExpress (MCE) Technical Support.
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