all-in- one SmartNuclease Lentivector Plasmid, 10 rxn, CASLV400PA-1, $695.00.

is a good option, by using 0.2ul/reaction, you can obtain about 50 reactions for cloning

https://www.systembio.com/downloads/Lenti-Cas9-prod-sheet.pdf

Hi, we have recently used Thermo's Gene Art CRISPR kit with great ease. Every thing you need comes in the kit (T7 polymerase, transfection reagents, confirmation assay), so it might be a good fit for your need. You just have to order your gRNA primers from IDT or Invitrogen. 

https://www.thermofisher.com/ca/en/home/life-science/genome-editing/geneart-crispr.html

Thank you very much Ahmad. I'll have a deep look at that.

Nevertheless, I was thinking more of a commercial kit with Cas9 protein, which you directly transfect to cells among with the sgRNA. These systems provide the oportunity to test sgRNA prior to transfection using PCR product as template, so that you are sure about sgRNA efficiency without transfection.

At any case, your information was really helpful. Thanks again

Thanks a lot Nicolas! That was quite what I was looking for. I was aware of this kit, but I needed some feedback on its user friendliness and efficiency, just to make sure it's worth paying for it.

Cheers!

Iker

Hi Iker,

I am wondering if you figured out a good commercial kit for your purpose? We are about to start CRISPR as well and are in the same situation as yours before.

Thanks!

Eugenie