Can you recommend a task-based material that can be used to compare with the currently used textbooks in English teaching?

Hello everyone, It was my first experience for a research. I plan to explore the differences in grammar teaching in the two kinds of books: one is task-based and one is currently used in a...

29 October 2020 5,495 4 View

Minimum clinically important difference (MCID) and sample size calculation for interventional studies

Hi all, Would you please advise me how to use the "minimum clinically important difference - MCID" to calculate sample size for interventional studies? Are there any web based sample size...

18 May 2017 4,868 8 View

Is it right to calculate O/C ratio from deconvolved peak areas of C1s peak?

I am performing graphene oxide reduction on SiO2 substrate. In this case, the XPS spectra will measure C1s, O1s and Si2p. Due to the O in SiO2, the O/C ratio cannot be simply calculated from C1s...

08 February 2017 7,301 6 View

How can I validate the purity of GST-tagged protein?

18 December 2016 1,769 9 View

Is there any paper about calculating mass loss of reinforcements in steel fiber reinforcement concrete by corrosion current density?

My research is about the effect of steel fiber reinforced concrete with transitional reinforcements. I could only find the relationship between corrosion current density and mass loss of...

11 December 2016 7,554 0 View

Why it keeps popping out 'sequence stats calculation failed' while completing the upload of sequence data to MG-RAST?

I was uploading the sequence data to the MG-RAST server, and the notice that sequence stats calculation failed kept popping out when the upload process was about to complete, meanwhile the...

02 August 2016 932 4 View

Does anyone have idea why shRNA and SiRNA show opposit effects on immune response on RAW264.7 cells?

Hello everybody, I am trying to produce ShRNA stable cell on RAW264.7. I succeed to get cells which is around 70-80% knockdown in puromycin selection condition. But the thing is when I measure the...

22 May 2016 7,188 6 View

Culturing EOL-1 cell line (a human eosinophil cell line)?

Dear all, I would like to ask for your advice with culturing culture Eol-1 cells (eosinophilic cell line). I resuscitated cells from frozen stock and suspended in RMPI 1640+ 10% FBS + 1% PS. After...

01 May 2016 6,653 8 View

Is it normal to have the immunofluorescent staining result like this? What's possibly wrong with it?

I used formalin-fixed paraffin-embedded liver tissue to do the immuofluorescent staining, but the result doesn't look good, can anyone tell me what is possibly wrong with it? I used 20% normal...

06 April 2016 942 9 View

Could anyone give me advice on PE & PE-Cy7 compensation in flow cytometry?

I used OneComp eBeads (eBioscience) to set up the compensation for PE & PE-Cy7. However, in the single PE staining tube, I couldn't achieve two distinct populations with similar mean values. I...

06 October 2015 3,326 6 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Should we reduce the standard unit of length to 0.31951540592 meter ?

The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...

27 February 2021 947 7 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

Methodological confusions regarding deductive and inductive reasoning, hypothesis and theory test?

Hi Colleagues- What do you think about the following methodological issues: 1. Is it methodologically wrong to formulate quantitative research questionnaire based on inductive reasoning? 2. Is it...

21 February 2021 4,634 6 View

What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View