Any comments on which is a better option for methylation microarray? Agilent platform or Illumina 450 BeadChip?

More Pooja Sagvekar's questions See All

Is there any good online workshop on advanced DEA models?

OR ANY study material with step by step analysis & its interpretation using the software?

01 January 2021 993 1 View

How do I get ConsistencyDupSNP.sh file required for genotype consistency quality control step of genotyping data obtained from GSA 24 v2.0 ?

Genotyping data is from Illumina Global screening array 24 v 2.0 and ConsistencyDupSNP.sh file contains list of pair of duplicated SNPs in GSA24 v 2.0.

18 December 2020 1,966 3 View

Can I get some important resources to analyse Photoluminescence Spectra?

I am trying to figure out what information can I get with the help of PL spectra and how to analyse it? How to do the defect analysis, if I am using two different material for thin film how will...

14 November 2020 2,453 6 View

Hazard value for malaria survival graph??

In an experiment untreated infected mice and infected treated mice observed. I recorded my data in terms of survived mice to 28 days in each group. How to calculate hazard value for treatment vs...

06 November 2020 5,548 1 View

How to analyze nominal/ordinal data in cases where you are testing multiple drugs/ infection against common risk factors?

chi-square or Fisher exact test I have a statistical question about chi-square and/or Fisher exact test. I have a cohort data studying the cause of infection in BRD with variables such as age,...

28 October 2020 2,589 1 View

What tips to follow while renaming a research paper in your repo for easier and quicker retrieval later during Ph.D research?

We often download tons of research paper related to our Ph.D research domain in their default filename format. After few days, recollecting and finding a particular paper becomes difficult. Any...

12 October 2020 2,425 3 View

Why will a very intense peak in XRD even beyond instrument detection limits, will appear in a Sample?

I recently submitted my samples in two different labs, I am getting three very intense peak in between 69-70 (2theta degrees); I want to know what could be the possible reason behind, is it normal...

13 September 2020 2,064 4 View

What kind of data ICPOES Perkin Elmer Optima 5300V ICP-OES provide with?

I recently submitted my samples to a laboratory for ICPOES, I am only provided with Standard Deviation and Mean data of one element out of two. I want to know if there is any spectra that is...

21 August 2020 2,428 2 View

How to check virulence of Fusarium oxysporum TR4?

Please add your suggestions for the following questions: 1. Can anyone suggest me the meaning of "fungus has lost its virulence"? 2. If I am able to observe micro and macroconidia under...

27 July 2020 1,099 1 View

Why do drug resistant cell lines show different morphology?

I have a HNSCC cell line resistant to a drug (2 different clones) showing different morphology than the parental cell line. Attached are phase contrast images of these cells. There are black dot...

15 July 2020 1,881 3 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Which detection method is better for an ELISA-based assay: colorimetric or fluorometric?

Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...

16 February 2021 8,389 3 View

Would bisulfide sequencing result be affected if the tissue is fixed by perfusion?

I am looking into DNA methylation of mice as an aging marker along with other assays and recently I added brain IF to the list of assays. For this, I need to fix the brain by transcardial...

09 February 2021 9,012 3 View

P53 detection in methylated and non methylated samples?

Hello everyone. I am about to search for the expression of genes including p53 in methylated and non methylated HPV samples. Should I extract DNA from the methylated samples and use SYBR green...

04 February 2021 2,749 3 View

What is your raw statistical treatment preference?

Hi, I have raw data from [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array that I want to process using the Expresso function for Affymetrix microarrays. My samples include tumor...

22 January 2021 1,527 3 View

Can someone explain why a methyl in phentolamine mesylate presents two correlations for 13C-HSQC, around the region it should appear?

Instead of appearing one single correlation of the signal of the carbon at 20.9 ppm (#11 in the attached figure), aligned to hydrogen at 2.29 ppm, it appeared two correlations (2,32 and 2,26 ppm)....

16 January 2021 4,774 2 View

Do I need to make a special plasmid for bisulfite methylation test?

I have an inducible expression of the enzyme in a newly derived bacterial strain, and methylation may be affected. I want to test methylation efficiency with a bisulfite kit. The kit is the EZ DNA...

06 January 2021 7,218 2 View

HPLC separation for methyl salicylate, camphor, thymol and menthol?

I have been recently trying to develop a method to separate the above compounds. Most methods tried were adapted from literature using RP C8 or C18 column with ACN/MeOH and buffered water at pH 2...

28 December 2020 4,426 6 View

How to read an HPLC report?

I have four HPLC graphs (for methyl ethyl ketone (MEK) and sulfuric acid and little organic monomers) and I want to analyze them. Is there anyone who can help me with this?

23 December 2020 6,180 3 View

Is there any GTEx like web-based tool that may predict DNA methylation associated gene expression?

I am looking for an online tool to see whether a certain methylated CpG site can affect the expression of a certain gene or not?

18 December 2020 7,575 1 View

Jonathan D Turner

Illumina 450k every time!

Jason Peter Ross

Illumina 450k is far more prevalent and the arrays have proved to be very robust.

Pooja Sagvekar

Many thanks, Jonathan and Jason. We are going for the same indeed.

Also, any particular kit that you would recommend to isolate DNA for the same? My samples are mammalian granulosa cells (not cultured)