TC-2 Tissue sectioner SORVALL?

I bought this tissue sectioner SORVALL TC-2, but I do not find the manual and I would like to know if someone uses this machine and can help me to understand how it works.

01 December 2020 550 2 View

Can someone help me to understand IGEM 2016 protocol to Bacillus subtilis transformation?

I'm trying to transform Bacillus subtilis to fluorescence, and I will use this IGEM protocol with medium MNGE, but there's a part that I can't understand. It says: "Measure the OD600 of your...

29 October 2020 2,043 1 View

Carbon content over 100 in a charcoal sample under treatment at 1200 C?

After elemental analysis of the samples, carbon content of the charcoal was 102 (several repetitions were made, but obtained the same values), the equipment was tested with other samples of wood...

02 September 2020 1,971 2 View

Can anyone help me with my contaminated human muscle cells in culture?

I am facing a problem with cell culture for weeks now. The media of the cell culture turnes turbid (white little spots, see picture) but stays reddish/pink. First this happened after 2 weeks of...

01 September 2020 1,498 9 View

Chi-Square Goodness of Fit: Can I hypothesize an expected outcome of 0 for one variable and 100 for the other variable?

If you have three variables A, B, C and you want to test whether the hypothesis is that everyone focuses on C, can the expected outcome in a chi-square goodness of fit test then be A: 0 B:0 and C:...

28 May 2020 2,008 6 View

Does anybody have a good Cytoplasmic Membrane stainning protocol?

11 February 2020 6,922 3 View

Which antibody for mouse p300 IP?

Which is the best Antibody to perform IP of endogenous mouse p300 IP. Please provide a figure or a citation in your answer. Thank you!

24 September 2019 8,482 2 View

How can I compute a factor (latent variable) knowing the variables that contribute?

Dear all, I have a data set with 7 variables. I have run a EFA and I got three factors. I want to obtain a numerical value for each factor computed from the variables that form it but I am not...

20 January 2019 1,933 3 View

Negative Results on Nanodrop

I am having negative results for my DNA/RNA extraction from stool samples on Nanodrop for Concentration, 260/280 and 260/230. This is the first time I'm having this problem and have tried to use...

17 June 2017 4,774 12 View

Does anyone have experience with heparinase I treatment of RNA preparations extracted from heparinized blood prior to library preparations/NGS?

Since heparin inhibits reverse transcriptase, heparinase I treatments prior to qRT-PCRs are recommended. NGS also uses reverse transcriptase, so I was wondering whether anyone has tried doing a...

30 May 2017 356 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View