Dear Pankaj Kumar Mishra, Essential oils can be potent in their capacity to control fungi and bacteria major pathogens of the root systems. I worked with Ginger and the use of cyanogenic plants can be very helpful in lowering inoculum potential. Oil radish was quite useful in that regard.
Check out Paret, M., et al. 2010. Effect of Plant essential oils on Ralstonia solancearum Race 4. Plant Disease 94(5):521-527.
Lemongrass and Plamarosa oils were bactericidal at fractions of a percent concentration and Eucalyptus was bacteriostatic.
Many have found cinnamon and cloves at 0.5 + 0.5% can give excellent fungal control on vegetable pathogens in foliar applications.
Since India is a champion on spices it might be interesting to assay curry.
good luck on your project and keep us posted. PRH
Hi Pankaj Kumar Mishra,
What follows you may already know, but it is worth emphasizing:
Since you wish to apply a seed treatment, you will probably have to use fairly low rates to assure that you do not inhibit germination of the seed. I am not sure exactly what you should start with but I would recommend using many different concentrations at first (rather than focusing on treatment replication). For instance, possibly use 2 or 3 replicates at 8-12 different rates. It is a good idea to separate your concentrations on a log scale, as well. Maybe you could use 0.001 ppm, 0.01 ppm, 0.1 ppm, 1 ppm, 10 ppm, 100 ppm, 1000 ppm, 10000 ppm. I would be very surprised if anything below 0.1 ppm had much of an effect. And anything above 1000 ppm may not be cost effective. The goal is to fit a dose-response curve to this data, and having more doses will help with this.
Also, the method in which you do these assays will influence what rates are effective. I presume you are soaking the seeds in the seed treatment and then sowing the seed in soil, on agar, or on Whatman filter paper to test germination. Or, perhaps you are soaking seed and then plating the seed to see if culturable pathogens grow from them. All of these factors will contribute to what rate is effective. I can not emphasize enough how important it is to use a broad range of rates if you can not find good dose information in the literature.
Best of luck,
Eli
Dear Pankaj Kumar Mishra
I used several plant extraction, neem, tee tree oil...and other but as foliar treatment to prevent insect vectors activity in viral transmission, colleges treat seed with garlic several plant extract …with or without Plant Growth–Promoting Rhizobacteria, results differ with the pathogen, plant spp. Soil pH, humidity…
Pls. find the attached files, may help you in selecting the best choice
https://www.researchgate.net/publication/236596962_Antimicrobial_activity_of_essential_oils_and_pure_oil_compounds_against_soilborne_pathogens_of_vegetables
Arun Arya, Analía Edith Perelló. 2010.Management of Fungal Plant Pathogens CABI,Pp.388. ISBN1845936043, 9781845936044
http://apsjournals.apsnet.org/doi/pdfplus/10.1094/PDIS-91-4-0375
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=15548&context=rtd
http://link.springer.com/article/10.1007/s10658-015-0789-2
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=15548&context=rtd
http://www.d3a.univpm.it/sites/www.d3a.univpm.it/files/d3a/Lavori%20scientifici/Mancini%20and%20Romanazzi,%202014%20Pest%20Management%20Science.pdf
http://link.springer.com/article/10.1007/s11104-008-9568-6
http://www.ijeit.com/Vol%204/Issue%2011/IJEIT1412201505_42.pdf
http://idosi.org/aejaes/jaes4(5)/6.pdf
Sangeetha Ganesan, Kurucheve Vadivel, Jayaraj Jayaraman 2015.Sustainable Crop Disease Management using Natural Products CABI,Pp.424. ISBN1780643233, 9781780643236
Regards
Prof. Houda Kawas
Data Antimicrobial activity of essential oils and pure oil compou...
Dear , Pankaj Kumar Mishra.
You also can find the following attached paper , they may be benefit you .
With my best greetings
Dear , Pankaj Kumar Mishra.
You can see the attached paper too.
Best regards
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