Hi,

I didn't use NTSYSPC for a long time. I am not sure whether I missed some steps or not. hope it will help you.

1. Prepare an Excel sheet includes your data. (You can use TXT files but I think xls is easier) I attached an example file for you. In example sheet, in first line, first number  is the number of your matrix (you need to check from the guide for the number of matrices and their means), second is number of the rows, third is number of columns and fourth number is 0 because there is no missing data in this data set.

2. open ntedit and import your xls file. You can name your samples in spreadsheet but I used to name them in this step (it's just a habit). Save your document as nts file.

3. open your file in NTSYS.

NTSYSpc:

Step 1:            Calculating similarity/dissimilary matrice

                       Similarity

                        SimQual

                        Input file {example.nts}

                        By rows {x}

                        Coefficient {JACCARD, DICE, etc}

                        Name Output file (examplejaccard i.e)

                        Compute

                       Step 2:            Performing hierarchical clustering using UPMGA

                       Clustering

                        SAHN

                        Input file {examplejaccard}

                        Output tree file {examplejaccardtree.nts}

                        Clustering method {UPGMA}

                        Compute

                        Click on the Dendrogram-bottom in the lower left corner of the window

                        Tree plot

                        Options

                        Plot Options

Hi,

thanks you, your instructions served me well. only that in addition to clarify the type of matrix and the number of colunmas and rows that have the same, I had to clarify the .txt file representing each if they were OTUS, Characters, etc.

Thanks for the help

Mylene, you may try to find more answers in the previous discussion at: https://www.researchgate.net/post/Any_advice_on_how_to_use_NTSYS_pc_202_software_for_genetic_diversity_analysis/1

There I explained step-by-step data handling.

Dear Branislav Siler,

Thanks for the suggestions,

How can i free download this software? Could you Please share it! Thank you