I'm doing time-lapse live-cell imaging of a monolayer of MDCKs for 24 hrs and 48 hrs. I'm monitoring cell migration over that period of time but, ideally, I would need to stop cell division in order to have the same number of cells in the microscope's field of view. I'm using DMEM supplemented with 1% FBS, which synchronize the cells by stopping division. However I still get division to some extent. Are there any alternative ways to stop division?