Dear friends, Hello, I have enjoyed the conversations very much and first of all I want to thank you for sharing. I am a PhD student in marine biology and decided to work in the subject of Microplastic ingestion by fishes in Southern Caspian Sea. At first part of my work, we are going to examine fish intestines to find any anthropocentric object and in second part, feed juvenile fish with mixtures of food and 0.5 to 5 micron Microplastic (MP) to find their possible cellular movements and effects on cell mechanisms of entrocytes. To find out, I want to use Transmission Electron Microscopy Technique to see weather MP is found in entrocytes of intestine or not and is there any effect on ultrastructure of entrocytes. I have written a proposal and presented it but some experts had doubt about efficiency and practical possibility of this method. I have used the Technique for normal tissues of different fish species but some people think in the EM images, MP will show up as empty spaces and they may be confused with other objects or artifacts. Therefore, I appreciate if you help me in this case. I need to know what you think about it. Happy 2016 and hope you have a nice holiday. Cheers, Zahra
sounds like a very cool project. I have only done work with SEM. Is there any reason you can't use SEM? Erik Zettler took some great pictures while mapping out the microbial community on plastics found in a variety of marine environments. I assume that you will supplement the images with spectroscopy. This article discusses methods for that as well. Hope this helps even a little bit!
با سلام
ببخشید من فارسی جواب دادم گفتم شاید راحتر باشید، بنده در حال حاضر در ایران هستم اگر سوالی دارید در مورد کارتون من در خدمت شما هستم البته اگه کاری از دست من برآید. شماره تماس من 09369552912 است اگر لازم میدونید تماس بگیرید خوشحال میشم اگر کمکتان کنم.
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James Austin Elliott, Thanks for your comment.
Erik R. Zettler and their team did a very amazing research and I will use SEM to find out any disturbance in apical surface of target cells but I need to see inside the cells and any possible damage of organelles as well.
Mehdi Hosseini سلام و روز بخیر
خیلی ممنونم. شما در زمینه میکروپلاستیک کار می کنید یا استفاده از میکروسکوپ گذاره ؟
با سلام
میکرو پلاستیک ترکیبات متنوعی داره و هر پژوهشگری از روش خاصی استفاده میکنه، نمونه برداری، فیلتراسیون، میکروسکوب و اسپکترومتر مهمترین روش در اندازه گیری ترکیبات میکروپلاستیک است. شما اول سعی کنید ترکیباتی که لازم است رو بشناسید بعد روش که شرایطش فراهم باشه رو انتخاب کنید، شما باید ستون خاصی برای این کار اماده کنید چون ستون ان با ستون دیگر الاینده ها مانند ترکیبات نفتی نیست، به هرحال مقاله من در زمینه میکروپلاستیک قبل از سال جدید منتشر خواهد شد میتونید از آن استفاده کنید.
موفق باشید
HI, how is it going?
I'm working with microplastics in fishes and I admire the size goal of 0,5 microns for microplastics.
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