I am trying to make a IF from isolated cardiomyocytes (mouse) for RYR2 but I don't get any pattern. After using several concentrations and protocols I have decided to buy a new antibody. Does anybody have any experience with RyR IF? do you have any advise for the choosing a new antibody?
I don have experience, but IF for RyR proved extremely complicated. I was using rat smooth muscle cells when I've done this, with a mouse Ab - thus my suggestions are useless to you....but I have had good luck with AbCam's Ab's for RyR's so I suggest starting there.
Alomone antibodies are pretty good for calcium channels, however i never tried RyR2
Thx for the answers. I am using an AbCam Ab for RyR2 but still no success. I have called them today to get detailed information about the protocol; may be there is a critical step that I am not taking into account.
I will update news and results soon. Thx again for your help.
In order not to leave this topic open I would like add that the antibody was not working until now for IF (several fixation protocols were tried with no success) but I have had positive results for WB.
We will keep trying!!! :)
Just if someone wants to try... IF for RyR worked!!! amazing pictures and results. Thx for the help.
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