I am going to use chick embryo for angiogenesis development assay. if i want to do a quantification, is there any free software that can be used? or can someone explain how to use ImageJ for it?
Select "Angiogenesis Analyzer" by clicking on the "toolsets" menu icon (>>) of the ImageJ tool bar. If the tool isn't visible in the list, download the file (here) in the "ImageJ/macros/toolset" folder. This can be done manually, or automatically using the "Luts Macros and Tools Updater".Oct 20, 2012
http://image.bio.methods.free.fr › I...
Angiogenesis Analyzer for ImageJ - Gilles Carpentier Research Web Site
The angiogenic effects can be measured by counting the number of blood vessels in a given area using a stereomicroscope. In a variation of the CAM assay, shell-less embryos are cultured in Petri dishes prior to applying the test substance (Auerbach et al.
https://www.ncbi.nlm.nih.gov › pmc
Current methods for assaying angiogenesis in vitro and in vivo
https://www.sartorius.com/en/products/live-cell-imaging-analysis/live-cell-analysis-software/incucyte-angiogenesis-analysis-software,kindly check this link.