Hi,

I think a bit of additional information is required to be able to answer your question.

What samples would you like to analyze (what is the matrix, what is the approximate protein concentration, etc.)?

Have you purified a set of proteins that you expect to be involved in the synthesis of nanoparticles (what kind of nanoparticles are you referring to, BTW?)?

A standard means to identify a protein is to digest it, for example with trypsin, and then generate mass and fragmentation data of the resulting peptides, by means of mass spectrometry.

These data can then be compared to that available in databases to enable identification of your protein(s) of interest.

MALDI can be used if you have a pure, or simple mixture of, protein(s), otherwise it is advised to use liquid chromatography coupled to ESI-MS, to first separate generated peptides before mass and fragmentation analysis.

Of course, in addition to a mass spectrometer, access to a database and search engine (software) is also of importance for successful identification of a (set of) protein(s).

Hope this helps!

Sir,

Protein content will be in th range of microgram. The matrix composition will be quinones, nitrate reductases, polyols, NADPH, proteins etc.

I have synthesized cobalt oxide nanoparticles. Proteins have not been purified. It is present along with nanoparticle solution.

Hello Ajuy,

are you performing protein assisted nanoparticle synthesis? To my knowledge, there are predefined proteins like ferritins or apoferritins involved in this method. I am wondering why you need to identify proteins that you allready know. Or did I understand something wrong?

Best, Murat

Murat Eravci Sir,

There is no predefined proteins. Proteins are produced by fungus during synthesis of nanoparticles