Hi everyone
Most papers are focused to study the lead oral bioaccesibility in urban soils with the Unified Bioaccessibility Method (UBM) from the Bioaccessibility Research Group of Europe (BARGE) (https://www.bgs.ac.uk/barge/home.html).
See for example
Roussel, H., Waterlot, C., Pelfrêne, A. et al. Arch Environ Contam Toxicol (2010) 58: 945. doi:10.1007/s00244-009-9425-5 http://link.springer.com/article/10.1007%2Fs00244-009-9425-5
Pelfrêne, A., Waterlot, C., Mazzuca, M. et al. Environ Geochem Health (2011) 33: 477. http://link.springer.com/article/10.1007%2Fs10653-010-9365-z
This method apply different reagents to simulate different conditions of saliva, gastric juice and Gastro-intestinal extraction....(several pHs). To make these different extractants... this method uses different reagents as inorganic solution (e.g. NaCl, NaH2PO4, etc.) but also uses pepsin, mucin, uric acid, etc... as organic solution.
I've some questions:
1. To apply this methodology, it's possible to make the extractions without organic solution (pepsina, urea, uric acid, etc.) and only make it extraction with a simulation of pH?
2. Anyone know an alternative methodology to investigate the bioaccessibility of lead and other metals in urban soils http://link.springer.com/article/10.1007/s11368-015-1304-2? TCLP method?
Thank you
dear Anders,
thank your for your comments and very good examples. That´s the purpos of my question. Motivating!!!
Peter
You can try the EPA method (EPA 9200.1-86), which relies solely on a Glycine (0.4M) solution at pH 1.5... I attach the PDF of the procedure.
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