Recombinant HSA is very expensive and I just need a few microliters for my dilution
One percent of bovine serum albumin (1% BSA w/v) works perfectly to store your aliquots.
Here is what I usually do with our lyophilized human-TNF-alpha.
While allowing human-TNF-alpha reach room temperature, I prepare a “fresh stock” of 1% BSA(w/v) in molecular grade purified water. Then I dissolve the human TNF-alpha in 1%BSA to make the stock solution (1mg/mL). From this stock, I make aliquots according to all my planned experiments (to avoid freeze-thaw cycles). All aliquots then are stored at -80°C. On the day of the experiment, I pick out one of my aliquots and dilute it with my media.
And from my personal experience! By using (1% BSA) did not cause any troubles.
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