What is the amplification program used in the thermocycler for PCR of the Sox C gene, and what are the specific temperature and time conditions for each cycle of the amplification program?
Find it in the article you took the primer sequences from.
Hello, I am currently attempting to obtain amplifications of SoxC from a species of onychophoran belonging to the Peripatidae family using primers designed from a sequence of its sister family,...
30 March 2024 1,551 4 View
What would be the usefulness of quantifying cDNA before conducting a PCR, and how could it influence the effectiveness and reproducibility of the obtained results?
20 March 2024 658 3 View
20 March 2024 4,626 4 View
In the gel that I made with the same cDNA but with different sets of primers, I had the results that are seen in the image in the 6 wells before the marker, it is the same primer starting with the...
15 September 2023 2,054 0 View
What concentrations of reagents are recommended to start standardizing an RT-PCR primer set?
22 August 2023 8,346 3 View
Hello, I've been having issues with my cDNA PCR which used to work previously. I'm working with cDNA from velvet worms, amplifying Cox1. Earlier, I had successfully amplified Cox1 with specific...
22 August 2023 6,262 4 View
I am doing a gradient PCR to achieve standardization of my primer set, however the results I have obtained from it are inconclusive. What do you recommend me to do? The image shows tm tests from...
17 May 2023 2,018 5 View
We want to perform a gradient PCR for our primers whose suggested Tm's are between 49.6°C and 50.7°C. We want to test with temperatures ranging from 47 to 55°C. Is this setup correct?
11 May 2023 762 3 View
why in my gel the loading dye is not distributing evenly? the loading dye is SafeView™ Classic
19 April 2023 9,755 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...
07 August 2024 648 0 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26
04 August 2024 9,919 4 View
How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?
02 August 2024 9,644 0 View